RFC5 antibody (N-Term)

Catalog No. ABIN6738219

This Rabbit Polyclonal antibody specifically detects RFC5 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Dog, Guinea Pig, Horse, Monkey and Rabbit.

Quick Overview for RFC5 antibody (N-Term) (ABIN6738219)

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Reactivity: Human, Dog, Guinea Pig, Horse, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RFC5 Antibody

Binding Specificity: N-Term

Specificity: Human RFC5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Guinea pig (100%) Mouse, Rat, Bovine, Pig, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human RFC5 (P40937, NP_031396). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Panda, Dog, Rabbit, Horse, Guinea pig (100%), Mouse, Rat, Bovine, Pig, Xenopus, Salmon, Pike, Zebrafish (92%), Opossum, Sablefish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RFC5

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Alternative Name: RFC5

Background: Name/Gene ID: RFC5

Synonyms: RFC5, Activator 1 subunit 5, A1 36 kDa subunit, Activator 1 36 kDa subunit, RF-C 36 kDa subunit, RFC, 36.5 kD subunit, RFC36, Replication factor C subunit 5

Gene ID: 5985

NCBI Accession: NP_031396

UniProt: P40937

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA