RFC5 antibody (AA 110-190)

Catalog No. ABIN7231006

The Rabbit Polyclonal anti-RFC5 antibody (ABIN7231006) specifically detects RFC5 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for RFC5 antibody (AA 110-190) (ABIN7231006)

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RFC5 Antibody

Binding Specificity: AA 110-190

Purpose: RFC5 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RFC5 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human RFC5 protein at AA range: 110-190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RFC5

Target: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Alternative Name: RFC5

Background: Rabbit Anti-RFC5 Polyclonal Antibody,Replication factor C subunit 5, Activator 1 36 kDa subunit, A1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, RF-C 36 kDa subunit, RFC36,RFC5 encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants.,RFC5

Molecular Weight: observerd band 37kDa

Gene ID: 5985

UniProt: P40937

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA