NCKAP1L antibody (AA 36-85)

Catalog No. ABIN6738379

The Rabbit Polyclonal anti-NCKAP1L antibody is suitable to detect NCKAP1L in samples from Human, Mouse, Cow, Dog, Rat, Horse, Rabbit, Bat and Monkey. It has been validated for WB.

Quick Overview for NCKAP1L antibody (AA 36-85) (ABIN6738379)

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Reactivity: Human, Mouse, Cow, Dog, Rat, Horse, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCKAP1L antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NCKAP1L Antibody

Binding Specificity: AA 36-85

Specificity: Human NCKAP1L

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human NCKAP1L (P55160, NP_005328). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Horse (100%), Elephant, Guinea pig (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NCKAP1L

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Alternative Name: NCKAP1L

Background: Name/Gene ID: NCKAP1L

Synonyms: NCKAP1L, HEM1, Hematopoietic protein 1, NCK-associated protein 1-like

Gene ID: 3071

NCBI Accession: NP_005328

UniProt: P55160

Pathways: Regulation of Actin Filament Polymerization