MAP3K2 antibody (AA 146-195)

Catalog No. ABIN6738543

The Rabbit Polyclonal anti-MAP3K2 antibody (ABIN6738543) specifically detects MAP3K2 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Horse, Monkey and Xenopus laevis samples.

Quick Overview for MAP3K2 antibody (AA 146-195) (ABIN6738543)

Target: MAP3K2 (Mitogen-Activated Protein Kinase Kinase Kinase 2 (MAP3K2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP3K2 Antibody

Binding Specificity: AA 146-195

Specificity: Human MAP3K2 / MEKK2

Predicted Reactivity: Percent identity by BLAST analysis: Dog, Bovine (100%) Rabbit, Guinea pig (92%) Mouse, Rat (85%) Horse (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of human MAP3K2 (Q9Y2U5, NP_006600). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine, Zebra finch, Xenopus (100%), Elephant, Rabbit, Guinea pig (92%), Mouse, Rat, Bat, Opossum (85%), Horse (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAP3K2

Target: MAP3K2 (Mitogen-Activated Protein Kinase Kinase Kinase 2 (MAP3K2))

Alternative Name: MAP3K2 / MEKK2

Background: Name/Gene ID: MAP3K2
Subfamily: MAP3K
Family: Protein Kinase

Synonyms: MAP3K2, MAP/ERK kinase kinase 2, MAPK/ERK kinase kinase 2, MAPKKK2, MEK kinase 2, MEKK 2, MEKK2, MEKK2B

Gene ID: 10746

NCBI Accession: NP_006600

UniProt: Q9Y2U5

Pathways: MAPK Signaling