LIG1 antibody (AA 578-627)

Catalog No. ABIN6738928

The Rabbit Polyclonal anti-LIG1 antibody has been validated for WB. It is suitable to detect LIG1 in samples from Human, Mouse, Rabbit, Dog, Guinea Pig, Monkey and Bat.

Quick Overview for LIG1 antibody (AA 578-627) (ABIN6738928)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Mouse, Rabbit, Dog, Guinea Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIG1 Antibody

Binding Specificity: AA 578-627

Specificity: Human LIG1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit (100%) Rat, Bovine, Horse, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa578-627 of human LIG1 (P18858, NP_000225). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Dog, Bat, Rabbit, Guinea pig (100%), Rat, Bovine, Horse, Pig, Stickleback (92%), Xenopus, Sea squirt, Nematode (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1 / DNA Ligase 1

Background: Name/Gene ID: LIG1

Synonyms: LIG1, DNA ligase I, DNA ligase 1, Ligase I, DNA, ATP-dependent

Gene ID: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA