LCK antibody (AA 74-123)

Catalog No. ABIN6738953

This Rabbit Polyclonal antibody specifically detects LCK in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Horse, Guinea Pig, Monkey and Bat.

Quick Overview for LCK antibody (AA 74-123) (ABIN6738953)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Horse, Guinea Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LCK Antibody

Binding Specificity: AA 74-123

Specificity: Human LCK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Sheep (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa74-123 of human LCK (P06239, NP_001036236). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Galago, Sheep (92%), Stickleback, Medaka, Pufferfish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: p56lck / LCK

Background: Name/Gene ID: LCK
Subfamily: Src
Family: Protein Kinase

Synonyms: LCK, Lck kinase, LSK, p56-LCK, Pp58lck, Tyrosine-protein kinase Lck, p56lck, Protein YT16, Proto-oncogene Lck, YT16

Gene ID: 3932

NCBI Accession: NP_001036236

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling