SPO11 antibody (AA 110-159)

Catalog No. ABIN6739020

This Rabbit Polyclonal antibody specifically detects SPO11 in WB. It exhibits reactivity toward Human, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio), Bat, Monkey and Pig.

Quick Overview for SPO11 antibody (AA 110-159) (ABIN6739020)

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Reactivity: Human, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio), Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPO11 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPO11 Antibody

Binding Specificity: AA 110-159

Specificity: Human SPO11

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Pig, Guinea pig (100%) Mouse, Rat, Rabbit, Horse (92%) Chicken (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa110-159 of human SPO11 (Q9Y5K1, NP_036576). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Bat, Pig, Opossum, Guinea pig (100%), Mouse, Rat, Rabbit, Horse, Zebra finch, Platypus (92%), Turkey, Chicken (84%), Stickleback (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPO11

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Alternative Name: SPO11

Background: Name/Gene ID: SPO11

Synonyms: SPO11, Cancer/testis antigen 35, CT35

Gene ID: 23626

NCBI Accession: NP_036576

UniProt: Q9Y5K1