alpha Tubulin antibody (N-Term)

Catalog No. ABIN364374

This anti-alpha Tubulin antibody is a Mouse Monoclonal antibody detecting alpha Tubulin in WB, ELISA, IHC, IF and IHC (p). Suitable for Human.

Quick Overview for alpha Tubulin antibody (N-Term) (ABIN364374)

Target: alpha Tubulin (TUBA1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha Tubulin Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Recognizes alpha tubulin of various species. Recognizes a N-terminal structural domain of alpha tubulin. Reacts with peptides that correspond to position alpha (65-79) of Tubulin, band at 50kD.

Purification: Ammonium sulfate precipitation

Immunogen: Peptide corresponding to the position alpha (65-79) of Tubulin.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: TUBA1 / Tubulin Alpha 1

Background: Name/Gene ID: TUBA1

Synonyms: TUBA1

Pathways: Microtubule Dynamics