NKX3-1 antibody (C-Term)

Catalog No. ABIN6740283

The Rabbit Polyclonal anti-NKX3-1 antibody has been validated for WB. It is suitable to detect NKX3-1 in samples from Human, Cow, Dog, Guinea Pig and Monkey.

Quick Overview for NKX3-1 antibody (C-Term) (ABIN6740283)

Target: NKX3-1 (NK3 Homeobox 1 (NKX3-1))

Reactivity: Human, Cow, Dog, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NKX3-1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NKX3-1 Antibody

Binding Specificity: C-Term

Specificity: Human NKX3-1

Predicted Reactivity: Percent identity by BLAST analysis: Dog (85%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NKX3-1 (Q99801, NP_006158). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NKX3-1

Target: NKX3-1 (NK3 Homeobox 1 (NKX3-1))

Alternative Name: NKX3-1

Background: Name/Gene ID: NKX3-1

Synonyms: NKX3-1, BAPX2, NK homeobox, family 3, A, NK3 homeobox 1, NKX3, NKX3.1, Homeobox protein Nkx-3.1, NKX3A

Gene ID: 4824

NCBI Accession: NP_006158

UniProt: Q99801

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Endopeptidase Activity, Positive Regulation of Response to DNA Damage Stimulus