NKX3-1 antibody (AA 92-224)

Catalog No. ABIN6940210

The Mouse Monoclonal anti-NKX3-1 antibody (Clone NKX3-1-2836) (ABIN6940210) specifically detects NKX3-1 in IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for NKX3-1 antibody (AA 92-224) (ABIN6940210)

Target: NKX3-1 (NK3 Homeobox 1 (NKX3-1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX3-1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: NKX3-1-2836

Product Details anti-NKX3-1 Antibody

Binding Specificity: AA 92-224

Purpose: NKX3.1 (Metastatic Prostate Adenocarcinoma Marker) Antibody

Specificity: NKX3.1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21.2, which frequently undergoes a loss of heterozygosity. NKX3.1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3.1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3.1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 92-224) of human NKX3.1 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Highly expressed in the prostate and at a lower level in the testis.

Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NKX3-1

Target: NKX3-1 (NK3 Homeobox 1 (NKX3-1))

Alternative Name: NKX3-1

Background:

NK3 Homeobox 1, Homeobox Protein NK-3 Homolog A, NKX3-1, NKX3A, NK3 Transcription Factor Related, Locus 1 (Drosophila), NK3 Transcription Factor Related, Locus 1, NK Homeobox (Drosophila), Family 3, A, NK3 Transcription Factor Homolog A, NK Homeobox, Family 3, A, Homeobox Protein Nkx-3.1, BAPX2, NKX3

Highly expressed in the prostate and at a lower level in the testis.

Molecular Weight: 35kDa

Gene ID: 4824, 55999

UniProt: Q99801

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Endopeptidase Activity, Positive Regulation of Response to DNA Damage Stimulus