ECD/SGT1 antibody (AA 72-121)

Catalog No. ABIN6740323

The Rabbit Polyclonal anti-ECD/SGT1 antibody has been validated for WB. It is suitable to detect ECD/SGT1 in samples from Human, Goat, Horse, Rabbit, Cow, Pig, Dog, Guinea Pig and Monkey.

Quick Overview for ECD/SGT1 antibody (AA 72-121) (ABIN6740323)

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Reactivity: Human, Goat, Horse, Rabbit, Cow, Pig, Dog, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECD/SGT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ECD/SGT1 Antibody

Binding Specificity: AA 72-121

Specificity: Human ECD

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Goat, Rabbit, Horse, Pig, Guinea pig (100%) Mouse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human ECD (O95905, NP_009196). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Goat, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Bat (93%), Mouse, Rat, Hamster, Opossum, Platypus (92%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ECD/SGT1

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Alternative Name: ECD

Background: Name/Gene ID: ECD

Synonyms: ECD, HSGT1, GCR2, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2

Gene ID: 11319

NCBI Accession: NP_009196

UniProt: O95905

Pathways: Regulation of Carbohydrate Metabolic Process