Phospholipase D2 antibody (AA 467-516)

Catalog No. ABIN6740325

This Rabbit Polyclonal antibody specifically detects Phospholipase D2 in WB. It exhibits reactivity toward Human, Rat, Cow and Monkey.

Quick Overview for Phospholipase D2 antibody (AA 467-516) (ABIN6740325)

Target: Phospholipase D2 (PLD2)

Reactivity: Human, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phospholipase D2 Antibody

Binding Specificity: AA 467-516

Specificity: Human PLD2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine (100%) Pig (91%) Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa467-516 of human PLD2 (O14939, NP_002654). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bovine (100%), Bat, Pig (91%), Rat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Phospholipase D2

Target: Phospholipase D2 (PLD2)

Alternative Name: PLD2 / Phospholipase D2

Background: Name/Gene ID: PLD2
Family: Phospholipase

Synonyms: PLD2, Choline phosphatase 2, Phospholipase D2, HPLD2, PLD 2, PLD1C

Gene ID: 5338

NCBI Accession: NP_002654

UniProt: O14939

Pathways: RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling