Phospholipase D2 antibody (N-Term)

Catalog No. ABIN6740534

The Rabbit Polyclonal anti-Phospholipase D2 antibody has been validated for WB. It is suitable to detect Phospholipase D2 in samples from Human, Rat, Mouse, Cow, Horse, Dog, Rabbit and Guinea Pig.

Quick Overview for Phospholipase D2 antibody (N-Term) (ABIN6740534)

Target: Phospholipase D2 (PLD2)

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phospholipase D2 Antibody

Binding Specificity: N-Term

Specificity: Human PLD2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Rabbit, Horse (85%) Dog (84%) Guinea pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PLD2 (O14939, NP_002654). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Marmoset (92%), Galago, Bovine, Rabbit, Horse (85%), Dog, Bat (84%), Guinea pig (83%), Mouse, Rat (75%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Phospholipase D2

Target: Phospholipase D2 (PLD2)

Alternative Name: PLD2 / Phospholipase D2

Background: Name/Gene ID: PLD2
Family: Phospholipase

Synonyms: PLD2, Choline phosphatase 2, Phospholipase D2, HPLD2, PLD 2, PLD1C

Gene ID: 5338

NCBI Accession: NP_002654

UniProt: O14939

Pathways: RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling