UBE3B antibody (AA 720-769)

Catalog No. ABIN6740821

This Rabbit Polyclonal antibody specifically detects UBE3B in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Hamster, Monkey, Pig.

Quick Overview for UBE3B antibody (AA 720-769) (ABIN6740821)

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE3B Antibody

Binding Specificity: AA 720-769

Specificity: Human UBE3B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa720-769 of human UBE3B (Q7Z3V4, NP_569733). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Stickleback, Zebrafish (92%), Drosophila (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE3B

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Alternative Name: UBE3B

Background: Name/Gene ID: UBE3B

Synonyms: UBE3B, Ubiquitin-protein ligase E3B, Ubiquitin protein ligase E3B

Gene ID: 89910

NCBI Accession: NP_569733

UniProt: Q7Z3V4