NEK3 antibody (AA 108-157)

Catalog No. ABIN6741054

This anti-NEK3 antibody is a Rabbit Polyclonal antibody detecting NEK3 in WB. Suitable for Human, Cow, Guinea Pig, Horse and Monkey.

Quick Overview for NEK3 antibody (AA 108-157) (ABIN6741054)

Target: NEK3 (NIMA related kinase 3 (NEK3))

Reactivity: Human, Cow, Guinea Pig, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NEK3 Antibody

Binding Specificity: AA 108-157

Specificity: Human NEK3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Horse, Guinea pig (100%) Mouse, Rat, Pig, Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human NEK3 (P51956, NP_002489). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Elephant, Bovine, Horse, Opossum, Guinea pig (100%), Mouse, Rat, Bat, Pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (92%), Rice (90%), Lizard, Stickleback (85%), Grape (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NEK3

Target: NEK3 (NIMA related kinase 3 (NEK3))

Alternative Name: NEK3

Background: Name/Gene ID: NEK3
Subfamily: NEK
Family: Protein Kinase

Synonyms: NEK3, HSPK 36, HSPK36, NIMA-related kinase 3, NimA-related protein kinase 3, Hydroxyalkyl-protein kinase, Phosphorylase B kinase kinase, Glycogen synthase A kinase

Gene ID: 4752

NCBI Accession: NP_002489

UniProt: P51956