MED8 antibody (N-Term)

Catalog No. ABIN6741137

This Rabbit Polyclonal antibody specifically detects MED8 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Hamster, Monkey and Pig.

Quick Overview for MED8 antibody (N-Term) (ABIN6741137)

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED8 Antibody

Binding Specificity: N-Term

Specificity: Human MED8

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human MED8 (Q96G25-2, NP_443109). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%), Opossum, Platypus, Xenopus (92%), Zebrafish (85%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED8

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Alternative Name: MED8

Background: Name/Gene ID: MED8

Synonyms: MED8, ARC32, Mediator complex subunit 8

Gene ID: 112950

NCBI Accession: NP_443109

UniProt: Q96G25

Pathways: Regulation of Lipid Metabolism by PPARalpha