Crossover junction endonuclease EME1 (EME1) (N-Term) antibody

Catalog No. ABIN6741209

The Rabbit Polyclonal anti-Crossover junction endonuclease EME1 antibody is suitable to detect Crossover junction endonuclease EME1 in samples from Human, Mouse, Pig, Cow, Horse and Monkey. It has been validated for WB.

Quick Overview for Crossover junction endonuclease EME1 (EME1) (N-Term) antibody (ABIN6741209)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human, Mouse, Pig, Cow, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Specificity: Human Eme1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Pig (100%) Rat, Dog, Guinea pig (92%) Horse (85%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human EME1 (Q96AY2, NP_689676). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Pig (100%), Gorilla, Rat, Panda, Dog, Guinea pig (92%), Rabbit, Horse (85%), Bovine (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1

Background: Name/Gene ID: EME1

Synonyms: EME1, MMS4, MMS4L, MMS4 homolog, SLX2A, HMMS4

Gene ID: 146956

NCBI Accession: NP_689676

UniProt: Q96AY2