RHOC antibody (AA 35-84)

Catalog No. ABIN6741373

Product Details anti-RHOC Antibody

Target: RHOC (Ras Homolog Gene Family, Member C (RHOC))

Binding Specificity: AA 35-84

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Zebrafish (Danio rerio), Horse, Drosophila melanogaster, Insect, Monkey, Pig, Xenopus laevis, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOC antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human RHOC

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human RHOC (P08134, NP_786886). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Seabass, Catfish, Smelt, Sablefish, Stickleback, Pike, Zebrafish, Drosophila, Mosquito, Slime mold, Nematode (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RHOC

Target: RHOC (Ras Homolog Gene Family, Member C (RHOC))

Alternative Name: RHOC (RHOC Products)

Synonyms: ARHC antibody, LOC100221682 antibody, ARH9 antibody, H9 antibody, RHOH9 antibody, AI324259 antibody, Arh9 antibody, Arhc antibody, CRHOC antibody, ras homolog family member C L homeolog antibody, ras homolog family member C antibody, rhoc.L antibody, RHOC antibody, rhoc antibody, Rhoc antibody

Background: Name/Gene ID: RHOC

Synonyms: RHOC, ARHC, Rho cDNA clone 9, RhoC GTPase, RHOH9, Small GTP binding protein RhoC, ARH9, H9, Oncogene RHO H9, Ras homolog family member C, RAS-related homolog 9

Gene ID: 389

NCBI Accession: NP_786886

UniProt: P08134

Pathways: WNT Signaling, Cell-Cell Junction Organization