UBE2K antibody (AA 36-85)

Catalog No. ABIN6741412

This anti-UBE2K antibody is a Rabbit Polyclonal antibody detecting UBE2K in WB. Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit, Guinea Pig, Chicken, Xenopus laevis, Pig, Monkey, Hamster, Bat and Drosophila melanogaster.

Quick Overview for UBE2K antibody (AA 36-85) (ABIN6741412)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit, Guinea Pig, Chicken, Xenopus laevis, Pig, Monkey, Hamster, Bat, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2K antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2K Antibody

Binding Specificity: AA 36-85

Specificity: Human UBE2K / HIP2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human UBE2K (P61086, NP_005330). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Xenopus, Salmon, Zebrafish, Drosophila (100%), Beetle (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2K

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: UBE2K / LIG

Background: Name/Gene ID: UBE2K

Synonyms: UBE2K, E2-25K, E2(25K), HIP2, HYPG, LIG, UBC1, Ubiquitin-protein ligase, HIP-2, Ubiquitin carrier protein

Gene ID: 3093

NCBI Accession: NP_005330

UniProt: P61086