CDC25C antibody (N-Term)

Catalog No. ABIN6741488

The Rabbit Polyclonal anti-CDC25C antibody has been validated for WB. It is suitable to detect CDC25C in samples from Human, Mouse, Rat, Chicken, Rabbit, Cow, Guinea Pig, Horse, Monkey, Pig and Sheep.

Quick Overview for CDC25C antibody (N-Term) (ABIN6741488)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Cow, Guinea Pig, Horse, Monkey, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC25C Antibody

Binding Specificity: N-Term

Specificity: Human CDC25C

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse (91%) Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human CDC25C (P30307, NP_073720). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Sheep, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%), Hamster (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25C

Background: Name/Gene ID: CDC25C
Subfamily: CDC25
Family: Protein Phosphatase

Synonyms: CDC25C, CDC25, CDC25C phosphatase, CDC25Hu3, Cell division cycle 25C, Phosphotyrosine phosphatase, PPP1R60, M-phase inducer phosphatase 3, Mitosis inducer CDC25

Gene ID: 995

NCBI Accession: NP_073720

UniProt: P30307

Pathways: Cell Division Cycle, M Phase