E2F1 antibody (AA 107-156)

Catalog No. ABIN6741507

The Rabbit Polyclonal anti-E2F1 antibody has been validated for WB. It is suitable to detect E2F1 in samples from Human, Mouse, Rat, Monkey, Dog, Guinea Pig, Rabbit, Horse, Bat, Pig and Chicken.

Quick Overview for E2F1 antibody (AA 107-156) (ABIN6741507)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse, Rat, Monkey, Dog, Guinea Pig, Rabbit, Horse, Bat, Pig, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-E2F1 Antibody

Binding Specificity: AA 107-156

Specificity: Human E2F1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human E2F1 (Q01094, NP_005216). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Lizard (100%), Xenopus (85%), Monkey, Panda, Bovine, Turkey, Platypus (84%), Zebra finch (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: Name/Gene ID: E2F1

Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3

Gene ID: 1869

NCBI Accession: NP_005216

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy