NOTCH4 antibody (AA 371-420)

Catalog No. ABIN6741546

This Rabbit Polyclonal antibody specifically detects NOTCH4 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Cow, Horse, Guinea Pig and Pig.

Quick Overview for NOTCH4 antibody (AA 371-420) (ABIN6741546)

Target: NOTCH4 (Notch 4 (NOTCH4))

Reactivity: Human, Cow, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NOTCH4 Antibody

Binding Specificity: AA 371-420

Brand: IHC-plus™

Specificity: Human NOTCH4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Horse, Pig (100%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa371-420 of human NOTCH4 (Q99466-2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Bovine, Horse, Pig (100%), Monkey, Marmoset (92%), Gibbon, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Alternative Name: NOTCH4

Background: Name/Gene ID: NOTCH4

Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4

Gene ID: 4855

UniProt: Q99466

Pathways: Notch Signaling