PML antibody (AA 588-637)

Catalog No. ABIN6741549

This Rabbit Polyclonal antibody specifically detects PML in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig and Horse.

Quick Overview for PML antibody (AA 588-637) (ABIN6741549)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PML Antibody

Binding Specificity: AA 588-637

Brand: IHC-plus™

Specificity: Human PML

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%) Pig (92%) Horse, Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa588-637 of human PML (P29590). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Bovine (100%), Marmoset, Panda, Bat, Rabbit, Pig (92%), Horse, Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background: Name/Gene ID: PML
Family: Transcription factor

Synonyms: PML, PP8675, Promyelocytic leukemia, Protein PML, TRIM19, Promyelocytic leukemia protein, RING finger protein 71, MYL, RNF71

Gene ID: 5371

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus