T-Box 5 antibody (AA 174-223)

Catalog No. ABIN6741551

This anti-T-Box 5 antibody is a Rabbit Polyclonal antibody detecting T-Box 5 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Goat, Sheep, Bat, Hamster, Monkey, Pig, Chicken and Xenopus laevis.

Quick Overview for T-Box 5 antibody (AA 174-223) (ABIN6741551)

Target: T-Box 5 (TBX5)

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Goat, Sheep, Bat, Hamster, Monkey, Pig, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Box 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-T-Box 5 Antibody

Binding Specificity: AA 174-223

Brand: IHC-plus™

Specificity: Human TBX5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Chicken, Xenopus, Zebrafish (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa174-223 of human TBX5 (Q99593, NP_000183). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Dolphin, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Newt, Stickleback, Medaka, Zebrafish (100%), Sea squirt (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for T-Box 5

Target: T-Box 5 (TBX5)

Alternative Name: TBX5

Background: Name/Gene ID: TBX5

Synonyms: TBX5, Hos, T-box protein 5, T-box 5

Gene ID: 6910

NCBI Accession: NP_000183

UniProt: Q99593