NPY1R antibody (AA 180-229)

Catalog No. ABIN462232

This Rabbit Polyclonal antibody specifically detects NPY1R in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Horse and Monkey.

Quick Overview for NPY1R antibody (AA 180-229) (ABIN462232)

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Reactivity: Human, Mouse, Rat, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NPY1R Antibody

Binding Specificity: AA 180-229

Brand: IHC-plus™

Specificity: Human NPY1R

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Sheep, Dog, Bovine, Rabbit, Pig, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human NPY1R (P25929, NP_000900). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Sheep, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig (92%), Hamster (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NPY1R

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Alternative Name: NPY1R

Background: Name/Gene ID: NPY1R
Subfamily: Neuropeptide
Family: GPCR

Synonyms: NPY1R, NPYY1, NPY-Y1 receptor, Neuropeptide Y receptor type 1, PY receptor Y1, Neuropeptide Y receptor Y1, Neuropeptide Y-Y1 receptor, Neuropeptide Y1 receptor, NPY1-R, NPYR

Gene ID: 4886

NCBI Accession: NP_000900

UniProt: P25929

Pathways: Negative Regulation of Hormone Secretion, Feeding Behaviour