UBE2L3 antibody (C-Term)

Catalog No. ABIN6741599

This Rabbit Polyclonal antibody specifically detects UBE2L3 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Chicken, Xenopus laevis, Pig, Bat and Monkey.

Quick Overview for UBE2L3 antibody (C-Term) (ABIN6741599)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Chicken, Xenopus laevis, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2L3 Antibody

Binding Specificity: C-Term

Specificity: Human UBE2L3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Pig, Guinea pig, Chicken, Xenopus (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human UBE2L3 (P68036, NP_003338). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Xenopus, Frog (100%), Hamster, Catfish, Salmon, Zebrafish (92%), Stickleback, Drosophila, Beetle (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UBE2L3 / UBCH7

Background: Name/Gene ID: UBE2L3

Synonyms: UBE2L3, E2-F1, L-UBC, UBCH7, UBCE7, Ubiquitin carrier protein L3, UbcM4, Ubiquitin-protein ligase L3

Gene ID: 7332

NCBI Accession: NP_003338

UniProt: P68036