MAS1 antibody (AA 187-236)

Catalog No. ABIN6741611

This Rabbit Polyclonal antibody specifically detects MAS1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Hamster and Monkey.

Quick Overview for MAS1 antibody (AA 187-236) (ABIN6741611)

Target: MAS1 (MAS1 Oncogene (MAS1))

Reactivity: Human, Mouse, Rat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAS1 Antibody

Binding Specificity: AA 187-236

Brand: IHC-plus™

Specificity: Human MAS1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa187-236 of human MAS1 (P04201, NP_002368). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Hamster, Dolphin (100%), Bovine (92%), Panda, Opossum, Guinea pig (85%), Galago (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAS1

Target: MAS1 (MAS1 Oncogene (MAS1))

Alternative Name: MAS1 / MAS

Background: Name/Gene ID: MAS1
Subfamily: Orphan-A
Family: GPCR

Synonyms: MAS1, Mas proto-oncogene, Proto-oncogene Mas, MAS, MAS1 oncogene

Gene ID: 4142

NCBI Accession: NP_002368

UniProt: P04201

Pathways: ACE Inhibitor Pathway, Regulation of Carbohydrate Metabolic Process