IDH1 antibody (AA 290-339)

Catalog No. ABIN6741619

This Rabbit Polyclonal antibody specifically detects IDH1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Monkey, Horse, Guinea Pig, Cow, Rabbit, Sheep, Pig, Hamster, Chicken and Xenopus laevis.

Quick Overview for IDH1 antibody (AA 290-339) (ABIN6741619)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse, Guinea Pig, Cow, Rabbit, Sheep, Pig, Hamster, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IDH1 Antibody

Binding Specificity: AA 290-339

Brand: IHC-plus™

Specificity: Human IDH1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa290-339 of human IDH1 (Q8LFC0, NP_005887). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Beetle (100%), Panda, Armadillo, Rice, Arabidopsis (92%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1 / IDH

Background: Name/Gene ID: IDH1

Synonyms: IDH1, IDCD, IDP, IDPC, PICD, NADP(+)-specific ICDH, IDH, Oxalosuccinate decarboxylase

Gene ID: 3417

NCBI Accession: NP_005887

UniProt: O75874

Pathways: Warburg Effect