MAPK12 antibody (AA 35-84)

Catalog No. ABIN6741665

The Rabbit Polyclonal anti-MAPK12 antibody has been validated for WB. It is suitable to detect MAPK12 in samples from Human, Mouse, Rat, Rabbit, Guinea Pig, Cow, Monkey, Horse, Pig and Xenopus laevis.

Quick Overview for MAPK12 antibody (AA 35-84) (ABIN6741665)

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Cow, Monkey, Horse, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAPK12 Antibody

Binding Specificity: AA 35-84

Specificity: Human MAPK12 / ERK5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig, Xenopus (100%) Dog, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human MAPK12 (P53778, NP_002960). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Xenopus, Catfish (100%), Gibbon (93%), Chimpanzee, Sheep, Elephant, Dog, Bat, Chicken, Platypus, Lizard, Salmon, Stickleback, Zebrafish (92%), Goat, Turkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAPK12

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Alternative Name: MAPK12 / ERK6 / SAPK3

Background: Name/Gene ID: MAPK12
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK12, ERK5, ERK6, ERK-6, MAP kinase 12, MAPK 12, MAP kinase p38 gamma, SAPK3, p38-gamma, p38GAMMA, SAPK-3, ERK3, PRKM12

Gene ID: 6300

NCBI Accession: NP_002960

UniProt: P53778

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins