RAGE antibody (AA 71-120)

Catalog No. ABIN6741735

The Rabbit Polyclonal anti-RAGE antibody has been validated for WB. It is suitable to detect RAGE in samples from Human, Mouse, Rat, Cow, Guinea Pig, Rabbit and Monkey.

Quick Overview for RAGE antibody (AA 71-120) (ABIN6741735)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAGE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAGE Antibody

Binding Specificity: AA 71-120

Specificity: Human AGER

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit (100%) Dog (92%) Pig, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human AGER (Q15109, NP_751947). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Rabbit (100%), Dog, Bat, Horse, Pig, Opossum (92%), Elephant, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: AGER / RAGE

Background: Name/Gene ID: AGER

Synonyms: AGER, RAGE, RAGE isoform NtRAGE-delta, RAGE isoform sRAGE-delta

Gene ID: 177

NCBI Accession: NP_751947

UniProt: Q15109

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins