SGK1 antibody (AA 71-120)

Catalog No. ABIN6741807

The Rabbit Polyclonal anti-SGK1 antibody has been validated for WB. It is suitable to detect SGK1 in samples from Human, Mouse, Rat, Cow, Dog, Monkey, Rabbit, Chicken, Guinea Pig, Horse, Bat, Hamster and Pig.

Quick Overview for SGK1 antibody (AA 71-120) (ABIN6741807)

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey, Rabbit, Chicken, Guinea Pig, Horse, Bat, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SGK1 Antibody

Binding Specificity: AA 71-120

Specificity: Human SGK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Chicken (100%) Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human SGK1 (O00141, NP_005618). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Xenopus, Zebrafish (92%), Stickleback (85%), Orangutan, Lizard, Seabass, Salmon (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Alternative Name: SGK1 / SGK

Background: Name/Gene ID: SGK1
Subfamily: SGK
Family: Protein Kinase

Synonyms: SGK1, Hsgk, Hsgk1, SGK, Sgk1 variant i3

Gene ID: 6446

NCBI Accession: NP_005618

UniProt: O00141

Pathways: MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway