AGO1 antibody (N-Term)

Catalog No. ABIN6741837

The Rabbit Polyclonal anti-AGO1 antibody (ABIN6741837) specifically detects AGO1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Monkey, Cow, Horse, Rabbit, Guinea Pig, Pig, Xenopus laevis and Bat samples.

Quick Overview for AGO1 antibody (N-Term) (ABIN6741837)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Cow, Horse, Rabbit, Guinea Pig, Pig, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AGO1 Antibody

Binding Specificity: N-Term

Specificity: Human EIF2C1 / AGO1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human AGO1 (Q9UL18, NP_036331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Platypus, Xenopus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: AGO1 / EIF2C

Background: Name/Gene ID: AGO1

Synonyms: AGO1, Argonaute 1, EIF-2C 1, EIF2C1, GERP95, EIF2C, EIF2C 1, HAgo1, Q99, Argonaute1, Protein argonaute-1

Gene ID: 26523

NCBI Accession: NP_036331

UniProt: Q9UL18

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization