PPARA antibody (AA 179-228)

Catalog No. ABIN6741860

This anti-PPARA antibody is a Rabbit Polyclonal antibody detecting PPARA in WB. Suitable for Human, Mouse, Rat, Dog, Horse, Pig and Monkey.

Quick Overview for PPARA antibody (AA 179-228) (ABIN6741860)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPARA Antibody

Binding Specificity: AA 179-228

Specificity: Human PPARA / PPAR

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Goat (93%) Mouse, Rat, Horse, Pig (86%) Dog (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human Piezo (Q07869, NP_005027). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Goat, Hamster, Bovine (93%), Galago (92%), Mouse, Rat, Horse, Pig, Opossum (86%), Marmoset (85%), Panda, Dog, Platypus (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPAR alpha

Background: Name/Gene ID: PPARA
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARA, PPAR Alpha, PPARalpha, PPAR-alpha, NR1C1, HPPAR, PPAR

Gene ID: 5465

NCBI Accession: NP_005027

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C