beta-2 Microglobulin antibody (N-Term)

Catalog No. ABIN6741983

The Rabbit Polyclonal anti-beta-2 Microglobulin antibody has been validated for WB. It is suitable to detect beta-2 Microglobulin in samples from Human, Mouse, Rat, Cow and Sheep.

Quick Overview for beta-2 Microglobulin antibody (N-Term) (ABIN6741983)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human, Mouse, Rat, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-beta-2 Microglobulin Antibody

Binding Specificity: N-Term

Specificity: Human B2M / Beta-2-Microglobulin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Rabbit, Horse, Pig, Guinea pig (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human B2M (P61769, NP_004039). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon (100%), Monkey, Marmoset, Dog, Cat, Rabbit, Horse, Pig, Guinea pig (92%), Baboon, Galago, Mouse, Rat, Elephant, Bat (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M / Beta 2 Microglobulin

Background: Name/Gene ID: B2M

Synonyms: B2M, Beta 2 Microglobulin, Beta-2-microglobulin, Beta-2-microglobin

Gene ID: 567

NCBI Accession: NP_004039

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process