Nitrilase 1 antibody (AA 71-120)

Catalog No. ABIN6741988

The Rabbit Polyclonal anti-Nitrilase 1 antibody has been validated for WB. It is suitable to detect Nitrilase 1 in samples from Human, Dog, Guinea Pig, Horse, Pig, Bat and Monkey.

Quick Overview for Nitrilase 1 antibody (AA 71-120) (ABIN6741988)

Target: Nitrilase 1 (NIT1)

Reactivity: Human, Dog, Guinea Pig, Horse, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nitrilase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nitrilase 1 Antibody

Binding Specificity: AA 71-120

Specificity: Human NIT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Guinea pig (100%) Mouse, Rat, Bovine, Rabbit (92%) Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human NIT1 (Q86X76, NP_005591). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bat, Horse, Opossum, Guinea pig (100%), Mouse, Rat, Hamster, Bovine, Rabbit (92%), Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nitrilase 1

Target: Nitrilase 1 (NIT1)

Alternative Name: NIT1

Background: Name/Gene ID: NIT1

Synonyms: NIT1, Nitrilase 1, Nitrilase homolog 1

Gene ID: 4817

NCBI Accession: NP_005591

UniProt: Q86X76