RPA1 antibody (AA 215-264)

Catalog No. ABIN6742105

Product Details anti-RPA1 Antibody

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Binding Specificity: AA 215-264

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Sheep, Cow, Dog, Pig, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human RPA1 / RPA70

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human RPA1 (P27694, NP_002936). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Xenopus (100%), Marmoset, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Drosophila (92%), Zebrafish (91%), Salmon (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA70 / RPA1 (RPA1 Products)

Synonyms: HSSB antibody, MST075 antibody, REPA1 antibody, RF-A antibody, RP-A antibody, RPA70 antibody, Cb1-727 antibody, wu:fi14b08 antibody, zgc:55337 antibody, zgc:77092 antibody, RPA1 antibody, hssb antibody, mst075 antibody, repa1 antibody, rf-a antibody, rp-a antibody, rpa antibody, rpa70 antibody, LOC100230990 antibody, 5031405K23Rik antibody, 70kDa antibody, AA589576 antibody, AW557552 antibody, Rpa antibody, CG9633 antibody, D-RPA70 antibody, D-SSB antibody, DRP-A antibody, DmRPA antibody, Dmel\\CG9633 antibody, RPA antibody, RPA 70 antibody, RPA 70 kDa antibody, RpA70 antibody, Rpa-70 antibody, Ssb-70 antibody, dRP-A antibody, dmrpa1 antibody, i164 antibody, replication protein A1 antibody, replication protein A1, 70kDa antibody, replication protein A1 L homeolog antibody, Replication Protein A 70 antibody, RPA1 antibody, Rpa1 antibody, rpa1 antibody, rpa1.L antibody, RpA-70 antibody

Background: Name/Gene ID: RPA1

Synonyms: RPA1, HSSB, MST075, Replication factor A protein 1, Replication protein A1 (70kD), RF-A, RF-A protein 1, MSTP075, Replication protein A1, 70  kDa, RP-A, REPA1, RP-A p70, RPA70

Gene ID: 6117

NCBI Accession: NP_002936

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA