AES antibody (AA 143-192)

Catalog No. ABIN6742262

The Rabbit Polyclonal anti-AES antibody has been validated for WB. It is suitable to detect AES in samples from Human, Mouse, Rat, Cow, Guinea Pig, Dog, Xenopus laevis, Zebrafish (Danio rerio), Monkey and Chicken.

Quick Overview for AES antibody (AA 143-192) (ABIN6742262)

Target: AES (Amino-terminal Enhancer of Split (AES))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Xenopus laevis, Zebrafish (Danio rerio), Monkey, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AES antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AES Antibody

Binding Specificity: AA 143-192

Specificity: Human AES / GRG / Groucho

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Xenopus (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human AES (Q14CJ1, NP_945320). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Opossum, Guinea pig, Chicken, Xenopus, Frog (100%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AES

Target: AES (Amino-terminal Enhancer of Split (AES))

Alternative Name: AES / Groucho

Background: Name/Gene ID: AES
Family: Transcription factor

Synonyms: AES, AES-1, AES-2, Amino enhancer of split, ESP1, Groucho, GRG, Protein ESP1, TLE5, Protein GRG, gp130-associated protein GAM, GRG5

Gene ID: 166

NCBI Accession: NP_945320

UniProt: Q08117

Pathways: WNT Signaling, SARS-CoV-2 Protein Interactome