ZEB1 antibody (AA 108-157)

Catalog No. ABIN6742270

This anti-ZEB1 antibody is a Rabbit Polyclonal antibody detecting ZEB1 in WB. Suitable for Human, Mouse, Rat, Rabbit, Dog, Pig, Bat, Monkey, Guinea Pig, Chicken and Hamster.

Quick Overview for ZEB1 antibody (AA 108-157) (ABIN6742270)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Pig, Bat, Monkey, Guinea Pig, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZEB1 Antibody

Binding Specificity: AA 108-157

Specificity: Human ZEB1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Pig, Guinea pig, Chicken (100%) Bovine, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human ZEB1 (P37275, NP_110378). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Rabbit, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Bovine, Lizard, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IF, IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1 / AREB6

Background: Name/Gene ID: ZEB1
Family: Transcription factor

Synonyms: ZEB1, AREB6, BZP, FECD6, NIL-2-A zinc finger protein, TCF-8, Negative regulator of IL2, NIL2A, ZEB, ZFHEP, DELTAEF1, NIL-2-A, PPCD3, TCF8, Transcription factor 8, ZFHX1A

Gene ID: 6935

NCBI Accession: NP_110378

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation