BRCA1 antibody (AA 1151-1200)

Catalog No. ABIN6742370

The Rabbit Polyclonal anti-BRCA1 antibody has been validated for WB. It is suitable to detect BRCA1 in samples from Human and Monkey.

Quick Overview for BRCA1 antibody (AA 1151-1200) (ABIN6742370)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 1151-1200

Specificity: Human BRCA1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1151-1200 of human BRCA1 (P38398, NP_009225). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Opossum (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: Name/Gene ID: BRCA1

Synonyms: BRCA1, BROVCA1, BRCC1, Breast cancer 1, early onset, PPP1R53, PSCP, PNCA4, RING finger protein 53, RNF53, BRCAI, Breast cancer 1 early onset, IRIS

Gene ID: 672

NCBI Accession: NP_009225

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus