PDX1 antibody (N-Term)

Catalog No. ABIN6742734

The Rabbit Polyclonal anti-PDX1 antibody has been validated for WB. It is suitable to detect PDX1 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Pig, Hamster and Monkey.

Quick Overview for PDX1 antibody (N-Term) (ABIN6742734)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDX1 Antibody

Binding Specificity: N-Term

Specificity: Human PDX1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Pig (100%) Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PDX1 (P52945, NP_000200). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Rabbit, Horse, Pig (100%), Galago, Panda, Bat, Opossum (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: PDX1 / PDX-1

Background: Name/Gene ID: PDX1

Synonyms: PDX1, Glucose-sensitive factor, Insulin upstream factor 1, IPF1, Insulin promoter factor 1, IPF-1, IUF-1, STF-1, GSF, IDX-1, Islet/duodenum homeobox-1, IUF1, MODY4, PDX-1

Gene ID: 3651

NCBI Accession: NP_000200

UniProt: P52945

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location