PIAS1 antibody (C-Term)

Catalog No. ABIN6742835

This Rabbit Polyclonal antibody specifically detects PIAS1 in WB. It exhibits reactivity toward Human, Horse, Cow, Bat, Monkey and Pig.

Quick Overview for PIAS1 antibody (C-Term) (ABIN6742835)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reactivity: Human, Horse, Cow, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIAS1 Antibody

Binding Specificity: C-Term

Specificity: Human PIAS1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human PIAS1 (O75925, NP_057250). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bat, Pig (100%), Galago, Elephant, Dog (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: PIAS1

Background: Name/Gene ID: PIAS1

Synonyms: PIAS1, AR interacting protein, DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, GU/RH-II, Gu-binding protein, GBP, ZMIZ3

Gene ID: 8554

NCBI Accession: NP_057250

UniProt: O75925

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C