ube3a antibody (AA 180-229)

Catalog No. ABIN6743132

Product Details anti-ube3a Antibody

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Binding Specificity: AA 180-229

Reactivity: Human, Rat, Zebrafish (Danio rerio), Bat, Monkey, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human UBE3A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Zebrafish (100%) Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human UBE3A (Q05086, NP_000453). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Zebrafish, Drosophila (100%), Galago, Mouse, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: E6AP / UBE3A (ube3a Products)

Synonyms: ANCR antibody, AS antibody, E6-AP antibody, EPVE6AP antibody, HPVE6A antibody, DDBDRAFT_0188760 antibody, DDBDRAFT_0302447 antibody, DDB_0188760 antibody, DDB_0302447 antibody, ube3a antibody, im:7140733 antibody, zgc:92173 antibody, 4732496B02 antibody, 5830462N02Rik antibody, A130086L21Rik antibody, Hpve6a antibody, mKIAA4216 antibody, As antibody, CG6190 antibody, Dmel\\CG6190 antibody, Dube3a antibody, UBE3A antibody, dUBE3A antibody, das antibody, dube3A antibody, dube3a antibody, MGC69536 antibody, UME3A antibody, ubiquitin protein ligase E3A antibody, ubiquitin-protein ligase E3A antibody, Ubiquitin protein ligase E3A antibody, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) S homeolog antibody, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) antibody, UBE3A antibody, ube3a antibody, LOC100194730 antibody, Ube3a antibody, ube3a.S antibody, Eint_040430 antibody

Background: Name/Gene ID: UBE3A

Synonyms: UBE3A, ANCR, E6-ap isoform-III, E6AP ubiquitin-protein ligase, EPVE6AP, HPVE6A, AS, CTCL tumor antigen se37-2, E6-AP, E6AP, Ubiquitin protein ligase E3A, Ubiquitin-protein ligase E3A

Gene ID: 7337

NCBI Accession: NP_000453

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway