AGO4 antibody (AA 611-660)

Catalog No. ABIN6743285

The Rabbit Polyclonal anti-AGO4 antibody is suitable to detect AGO4 in samples from Human, Rat, Dog, Guinea Pig, Rabbit, Monkey and Pig. It has been validated for WB.

Quick Overview for AGO4 antibody (AA 611-660) (ABIN6743285)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reactivity: Human, Rat, Dog, Guinea Pig, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AGO4 Antibody

Binding Specificity: AA 611-660

Specificity: Human EIF2C4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Pig, Guinea pig (100%) Mouse, Chicken (92%) Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa611-660 of human AGO4 (Q9HCK5, NP_060099). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Rabbit, Pig, Guinea pig (100%), Mouse, Opossum, Chicken, Platypus (92%), Rat, Turkey, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Alternative Name: AGO4 / EIF2C4

Background: Name/Gene ID: AGO4

Synonyms: AGO4, Argonaute4, EIF2C4, EIF2C 4, HAgo4, KIAA1567, Protein argonaute-4, Argonaute 4, EIF-2C 4

Gene ID: 192670

NCBI Accession: NP_060099

UniProt: Q9HCK5

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process