TUBB antibody (AA 36-85)

Catalog No. ABIN6743878

The Rabbit Polyclonal anti-TUBB antibody has been validated for WB. It is suitable to detect TUBB in samples from Human, Mouse, Rat, Rabbit, Pig, Chicken, Monkey, Dog, Zebrafish (Danio rerio), Hamster, Cow, Xenopus laevis, Horse, Guinea Pig and Bat.

Quick Overview for TUBB antibody (AA 36-85) (ABIN6743878)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Monkey, Dog, Zebrafish (Danio rerio), Hamster, Cow, Xenopus laevis, Horse, Guinea Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TUBB Antibody

Binding Specificity: AA 36-85

Specificity: Human TUBB / Tubulin, Beta

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human TUBB (P07437, NP_821133). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Xenopus, Salmon, Smelt, Stickleback, Medaka, Zebrafish (100%), Platypus, Sablefish, Drosophila (92%), Turkey, Slime mold, Rice, Nematode, Arabidopsis, Paramecium, Amoeba (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB / Beta Tubulin

Background: Name/Gene ID: TUBB

Synonyms: TUBB, Beta Tubulin, Beta 5-tubulin, Beta-4 tubulin, Beta1-tubulin, M40, Tubulin beta-5 chain, Beta Ib tubulin, Class I beta-tubulin, OK/SW-cl.56, TUBB5, Tubulin beta chain, Tubulin beta polypeptide, Tubulin beta-1 chain, Tubulin, beta class I, Tubulin, beta polypeptide

Gene ID: 203068

NCBI Accession: NP_821133

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase