PRAME antibody (AA 71-120)

Catalog No. ABIN6743941

This anti-PRAME antibody is a Rabbit Polyclonal antibody detecting PRAME in WB. Suitable for Human, Horse, Pig and Rabbit.

Quick Overview for PRAME antibody (AA 71-120) (ABIN6743941)

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reactivity: Human, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAME antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRAME Antibody

Binding Specificity: AA 71-120

Specificity: Human PRAME

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat (92%) Bovine, Pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human PRAME (P78395, NP_006106). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%), Gorilla, Mouse, Rat, Bovine (92%), Marmoset (91%), Hamster, Pig (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Alternative Name: PRAME

Background: Name/Gene ID: PRAME

Synonyms: PRAME, Cancer/testis antigen 130, CT130, MAPE, Opa-interacting protein 4, Opa-interacting protein OIP4, OIP-4, OIP4

Gene ID: 23532

NCBI Accession: NP_006106

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding