Nodal antibody (AA 35-84)

Catalog No. ABIN6744117

This Rabbit Polyclonal antibody specifically detects Nodal in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Horse, Guinea Pig and Rabbit.

Quick Overview for Nodal antibody (AA 35-84) (ABIN6744117)

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Reactivity: Human, Rat, Mouse, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nodal antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nodal Antibody

Binding Specificity: AA 35-84

Specificity: Human NODAL

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human NODAL (Q96S42, NP_060525). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (100%), Monkey, Elephant, Bovine (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nodal

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Alternative Name: NODAL

Background: Name/Gene ID: NODAL

Synonyms: NODAL, HTX5, Nodal homolog, Nodal, mouse, homolog, Nodal homolog (mouse)

Gene ID: 4838

NCBI Accession: NP_060525

UniProt: Q96S42

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity