SOD2 antibody (N-Term)

Catalog No. ABIN6744284

The Rabbit Polyclonal anti-SOD2 antibody has been validated for WB. It is suitable to detect SOD2 in samples from Human, Sheep, Dog, Rabbit, Cow, Guinea Pig, Monkey, Goat and Zebrafish (Danio rerio).

Quick Overview for SOD2 antibody (N-Term) (ABIN6744284)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Human, Sheep, Dog, Rabbit, Cow, Guinea Pig, Monkey, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SOD2 Antibody

Binding Specificity: N-Term

Specificity: Human SOD2 / MNSOD

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Guinea pig (100%) Mouse, Rat, Pig, Chicken (92%) Zebrafish (85%) Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SOD2 (P04179, NP_000627). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Goat, Elephant, Dog, Bovine, Rabbit, Opossum, Guinea pig, Grape (100%), Galago, Mouse, Rat, Horse, Pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Pike, Nematode (92%), Salmon, Tunicate (90%), Bat, Xenopus, Sablefish, Stickleback, Zebrafish, Sea squirt (85%), Smelt, Drosophila (84%), Frog (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: MNSOD / SOD2

Background: Name/Gene ID: SOD2

Synonyms: SOD2, IPOB, MVCD6, Indophenoloxidase B, Mn superoxide dismutase, MNSOD, Mangano-superoxide dismutase

Gene ID: 6648

NCBI Accession: NP_000627

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling