Cyclin D1 antibody (C-Term)

Catalog No. ABIN6744353

The Rabbit Polyclonal anti-Cyclin D1 antibody has been validated for WB. It is suitable to detect Cyclin D1 in samples from Human, Mouse, Rat, Monkey, Guinea Pig, Bat and Hamster.

Quick Overview for Cyclin D1 antibody (C-Term) (ABIN6744353)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat, Monkey, Guinea Pig, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cyclin D1 Antibody

Binding Specificity: C-Term

Specificity: Human CCND1 / Cyclin D1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Guinea pig (100%) Dog, Bovine, Horse, Chicken, Xenopus (90%) Zebrafish (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CCND1 (P24385, NP_444284). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Bat, Guinea pig (100%), Elephant, Panda, Dog, Bovine, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Zebrafish (90%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 / Cyclin D1

Background: Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

Gene ID: 595

NCBI Accession: NP_444284

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling