LDHB antibody (C-Term)

Catalog No. ABIN6744435

The Rabbit Polyclonal anti-LDHB antibody has been validated for WB, IHC and IHC (p). It is suitable to detect LDHB in samples from Human, Monkey, Dog, Rabbit and Chicken.

Quick Overview for LDHB antibody (C-Term) (ABIN6744435)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivity: Human, Monkey, Dog, Rabbit, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LDHB Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human LDHB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Chicken (100%) Mouse, Rat, Bovine, Horse, Pig (92%) Zebrafish (90%).

Purification: Affinity purified

Immunogen: Synthetic peptide from C-Terminus of human LDHB (P07195, NP_002291). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Ferret, Dolphin, Elephant, Panda, Dog, Rabbit, Opossum, Turkey, Chicken (100%), Galago, Mouse, Rat, Hamster, Bovine, Horse, Pig, Guinea pig (92%), Catfish (91%), Zebrafish (90%), Zebra finch (85%), Salmon (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB / LDH-B

Background: Name/Gene ID: LDHB

Synonyms: LDHB, LDH heart subunit, Lactate dehydrogenase H chain, Lactate dehydrogenase B, LDH-B, TRG-5, LDH-H, LDHBD

Gene ID: 3945

NCBI Accession: NP_002291

UniProt: P07195

Pathways: Warburg Effect