Tyrosinase-Related Protein 1 antibody (AA 396-445)

Catalog No. ABIN490060

This Rabbit Polyclonal antibody specifically detects Tyrosinase-Related Protein 1 in IHC, WB and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Goat, Horse, Rabbit, Sheep, Guinea Pig, Zebrafish (Danio rerio), Monkey, Chicken and Bat.

Quick Overview for Tyrosinase-Related Protein 1 antibody (AA 396-445) (ABIN490060)

Target: Tyrosinase-Related Protein 1 (TYRP1)

Reactivity: Human, Mouse, Rat, Dog, Goat, Horse, Rabbit, Sheep, Guinea Pig, Zebrafish (Danio rerio), Monkey, Chicken, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosinase-Related Protein 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Tyrosinase-Related Protein 1 Antibody

Binding Specificity: AA 396-445

Brand: IHC-plus™

Specificity: Human TYRP1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Goat, Rabbit, Horse, Guinea pig (100%) Bovine, Pig, Chicken (92%) Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human TYRP1 (P17643, NP_000541). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Goat, Panda, Dog, Cat, Bat, Rabbit, Horse, Guinea pig, Chicken, Platypus (100%), Elephant, Bovine, Pig, Opossum, Zebra finch, Lizard (92%), Xenopus (85%), Pufferfish, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: TYRP1 / gp75

Background: Name/Gene ID: TYRP1

Synonyms: TYRP1, B-PROTEIN, CAS2, CATB, Glycoprotein 75, Hstyrrp, gp75, OCA3, SHEP11, TRP-1, Tyrosinase-related protein 1, TYRP, TYRRP, Catalase B, DHICA oxidase, Melanoma antigen gp75

Gene ID: 7306

NCBI Accession: NP_000541

UniProt: P17643